FLAVONOIDS AND LIGNANS FROM THE LEAVES OF NEPETA MULTIFIDA (LAMIACEAE) AND THEIR BIOAC-TIVITY

A perennial plant Nepeta multifida L. (syn. Schizonepeta (L.) Briq.) is one of the most common species Lamiaceae family growing in Eastern Siberia and used traditional oriental medicine. The chemical composition N. has not been sufficiently studied. Chromatographic separation phenolic compounds leaves using column chromatography preparative HPLC resulted isolation sixteen compounds, including a new flavonoid identified as luteolin-7-O-(3′′,6′′-di-O-acetyl)-β-D-glucopyranoside. Known were O-glycosides luteolin apigenin, rosmarinic acid, salvianolic acids B, schizotenuin A. Quantitative analysis by HPLC-UV assay showed high content acid (8.36–35.71 mg/g), luteolin-7-O-glucuronide (2.03–14.18 mg/g) (5.29–9.56 mg/g). highest level was found flowering fruiting phases. Using Ellman’s spectrophotometric method, it that leaf extract some had antiacetylcholinesterase activity, glycosides being active concentration half-maximal enzyme inhibition (IC50) 29.03–58.36 μg/mL. Thus, result present study, contain various groups capable inhibiting activity acetylcholinesterase.